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RICARDO DOMINGUEZ + GARDEN OF EDEN: BETWEEN HUMAN AND ANIMAL

DIANE LUDIN 22 MAY 2014
CNSI ART|SCI GALLERY
QUANTITATIVE NANOSTRUCTURAL AND SINGLE-MOLECULE FORCE SPECTROSCOPY
BIOMOLECULAR ANALYSIS OF HUMAN-SALIVA-DERIVED EXOSOMES Garden of Eden was an installation
with multichannel projections and live
SHIVANI SHARMA / BOYD M. GILLESPIE / VISWANATHAN PALANISAMY / JAMES K. GIMZEWSKI performance that explores human and
animal entanglements, reversing the
Exosomes are naturally occurring nanoparticles with unique structure, surface biochemistry, and mechanical roles of humans and animals. Scenes
characteristics. These distinct nanometer-sized bioparticles are secreted from the surfaces of oral epithelial included an eerily human raven song
cells into saliva and are of interest as oral-cancer biomarkers. We use high-resolution AFM to show single-vesicle and dance, an address to the UN General
quantitative differences between exosomes derived from normal and oral cancer patient’s saliva. Assembly and a Socratic dialogue
featuring a cast of animals performing
Compared to normal exosomes (circular, 67.4 ( 2.9 nm), our findings indicate that cancer exosome populations are as writers and philosophers.
significantly increased in saliva and display irregular morphologies, increased vesicle size (98.3 ( 4.6 nm), and
higher intervesicular aggregation.

]At the single-vesicle level, cancer exosomes exhibit significantly (P < 0.05) increased CD63 surface densities. To
our knowledge, it represents the first report detecting single-exosome surface protein variations.

High-resolution AFM imaging of cancer saliva samples revealed discrete multivesicular bodies with intraluminal
exosomes enclosed. We discuss the use of quantitative, nanoscale ultrastructural and surface biomolecular analysis
of saliva exosomes at single-vesicle and single-protein-level sensitivities as a potentially new oral cancer
diagnostic.

ARTIST ART+ACTIVISM COLLABORATION COMMUNITY EDUCATION LECTURE PUBLICATION RESEARCH RESIDENCY ROBOTICS MIXER SYMPOSIUM |||| ARTIST ART+ACTIVISM COLLABORATION COMMUNITY EDUCATION LECTURE PUBLICATION RESEARCH RESIDENCY ROBOTICS MIXER SYMPOSIUM ||||
|| SPRING SUMMER FALL WINTER ||||| 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 ||||| UCLA CNSI DMA BEYOND ||||| ||||| SPRING SUMMER FALL WINTER ||||| 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 ||||| UCLA CNSI DMA BEYOND |